rat anti mycl  (Bioss)

Journal: Journal of Biological Chemistry

Article Title: Phosphoinositide Binding by the Disabled-1 PTB Domain Is Necessary for Membrane Localization and Reelin Signal Transduction

doi: 10.1074/jbc.m413356200

Figure Lengend Snippet: FIG. 6. Tyrosine phosphorylation of Disabled-1 mutants upon Reelin stimulation. Primary neurons were transduced with lentiviral particles expressing different Myc-tagged Dab1 constructs. The cells were exposed to mock or Reelin-conditioned media and then lysed. The virally expressed Dab1 was immunoprecipitated from the lysate using a polyclonal -Myc antibody, and then analyzed by Western blot for levels of phosphotyrosine and Myc. M, mock-conditioned media; R, Reelin-conditioned media. Vec, empty vector; WT, wild-type Dab1. Dab1 mutants are identified by their amino acid substitutions. The empty vector and 5F samples serve as negative controls. A, immunoprecipi- tated (IP) Western blot to analyze phosphorylation of the S114T/F158V NPXY binding mutant. B, immunoprecipitated Western blot to analyze phosphorylation of the K45E PI binding mutant.

Article Snippet: The cleared lysates were incubated with 5 g of a polyclonal rabbit -Myc antibody (Bethyl Labs) at 4 °C overnight.

Techniques: Phospho-proteomics, Transduction, Expressing, Construct, Immunoprecipitation, Western Blot, Plasmid Preparation, Binding Assay, Mutagenesis